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Hele Mount immunfluorescensfarvning af Neonatal Mouse Retina at undersøge Angiogenesis<em> In vivo</em
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JoVE Journal Neuroscienze
Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

Hele Mount immunfluorescensfarvning af Neonatal Mouse Retina at undersøge Angiogenesis<em> In vivo</em

DOI:
10.3791/50546-v

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08:47 min

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July 09, 2013

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, , ,
1Institute of Genetic Medicine,Newcastle University , 2UCL Institute of Ophthalmology,University College, London

Capitoli

  • 00:05Titolo
  • 00:56Enucleation and Fixation of Postnatal Eyes
  • 02:01Dissection of Postnatal Murine Retinas
  • 04:59Immuno/isolectin Staining of the Retinal Vasculature
  • 07:07Typical Staining of Endothelial Cell and Pericytes
  • 08:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

Den neonatale murine nethinden giver et godt karakteriseret fysiologisk model af angiogenese, der tillader undersøgelser af de roller, som forskellige gener eller narkotika, der modulerer angiogenese i en<em> In vivo</em> Kontekst. Immunfluorescensfarvning præcist visualisere det vaskulære plexus er afgørende for succes i disse typer af undersøgelser.

Tags

Keywords: AngiogenesisNeonatal Mouse RetinaVascular PlexusFluorescent StainingIsolectinVascular AntibodiesIn VivoBlood Vessel Formation2D StructureVascular DevelopmentDisease Modeling

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