Rivista
/
Biologia
/
Prostaglandin Extraction and Analysis in Caenorhabditis elegans
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

DOI:
10.3791/50447-v

•

08:50 min

•

June 25, 2013

•

, , ,
1Department of Pharmacology and Toxicology,University of Alabama at Birmingham, 2Department of Cell, Developmental, and Integrative Biology,University of Alabama at Birmingham

Capitoli

  • 00:05Titolo
  • 01:05Worm Culture
  • 03:28Prostaglandin Extraction
  • 06:14Prostaglandin Analysis
  • 07:15Results: Prostaglandin Extraction and Analysis in C. elegans
  • 08:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

In this paper, we describe an optimized procedure for extracting and analyzing prostaglandins and other eicosanoids from C. elegans using LC-MS/MS.

Tags

ProstaglandinCaenorhabditis ElegansEicosanoidLipid ExtractionLiquid Chromatography-mass SpectrometryTandem Mass SpectrometryMultiple Reaction MonitoringCollision-induced Decomposition

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Read Article