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Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling
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Medicina
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JoVE Journal Medicina
Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

11,941 Views

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16:43 min

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March 03, 2013

DOI:

10.3791/50218-v

DOI:
10.3791/50218-v

•

16:43 min
March 03, 2013

•

3 Views
, , , , , , ,
1Department of Biomedical Engineering,University of Virginia, 2Department of Biomedical Engineering,California Polytechnic State University, 3Office of Animal Welfare,University of Virginia, 4Department of Biomedical Engineering & Institute for Computational Medicine,Johns Hopkins University

Summary

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We demonstrate a novel arterial ligation model in murine spinotrapezius muscle, including a step-by-step procedure and description of required instrumentation. We describe the surgery and relevant outcome measurements relating to vascular network remodeling and functional vasodilation using intravital and confocal microscopy.

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Murine SpinotrapeziusArteriolar LigationMicrovascular FunctionMicrovascular RemodelingIntravital MicroscopyConfocal MicroscopySkeletal Muscle IschemiaRevascularizationFunctional VasodilationImmunostaining

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Cite this Article

Guendel, A. M., Martin, K. S., Cutts, J., Foley, P. L., Bailey, A. M., Mac Gabhann, F., Cardinal, T. R., Peirce, S. M. Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling. J. Vis. Exp. (73), e50218, doi:10.3791/50218 (2013).

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