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Isolatie en Cultuur van individuele myovezels en hun satelliet cellen van volwassen skeletspier
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
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Isolatie en Cultuur van individuele myovezels en hun satelliet cellen van volwassen skeletspier

61,283 Views

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11:57 min

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March 22, 2013

DOI:

10.3791/50074-v

DOI:
10.3791/50074-v

•

11:57 min
March 22, 2013

•

42 Views
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1Sprott Center for Stem Cell Research,Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine,University of Ottawa

Summary

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Isolatie en cultuur van myovezels is de gouden standaard<em> In vitro</em> Om de overgang van satelliet-cellen door middel van rust, activering en differentiatie te bestuderen. Belangrijk is dat de enige myofiber cultuur systeem bewaart de myofiber / stamcel vereniging, die is een essentieel onderdeel van de spier stamcel niche.

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Pasut, A., Jones, A. E., Rudnicki, M. A. Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle. J. Vis. Exp. (73), e50074, doi:10.3791/50074 (2013).

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