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同时脑电图，实时测量的啮齿动物的大脑皮质中的神经元活动的乳酸浓度与Optogenetic的操作
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex
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同时脑电图，实时测量的啮齿动物的大脑皮质中的神经元活动的乳酸浓度与Optogenetic的操作

DOI:
10.3791/4328-v

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10:45 min

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December 19, 2012

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, , ,
1Department of Veterinary & Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, WWAMI Medical Education Program,Washington State University

Capitoli

  • 00:05Titolo
  • 02:18Anesthesia, Stereotaxic Placement and Clearance of Skull
  • 03:20Cannulas, EMG Leads and EEG Leads
  • 04:55Assembly of Rodent Optogenetic Stimulation System
  • 06:42Measurement of EEG-defined Sleep and Lactate Concentration during Optogenetic Stimulation
  • 08:19Optogenetic Stimulation: EEG-defined Sleep and Lactate Concentration
  • 09:26Conclusion

Summary

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一个过程的描述，操纵大脑皮质锥体神经元的活动optogenetically而脑电图，肌电图，脑乳酸浓度监测。进行实验记录，电缆绳系小鼠，而他们自发的睡眠/觉醒周期。 optogenetic设备被组装在我们的实验室;记录设备是市售的。

Tags

Simultaneous ElectroencephalographyReal-time MeasurementLactate ConcentrationOptogenetic ManipulationNeuronal ActivityRodent Cerebral CortexGlucose UtilizationNon Rapid Eye Movement SleepCerebral MetabolismSlow WavesElectroencephalogramDepolarized/up StateHyperpolarized/down StateAction Potentials

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