Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Neuroscience

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex
 

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

Article DOI: 10.3791/4328-v 10:45 min December 19th, 2012
December 19th, 2012

Kapitel

Summary

A procedure is described for manipulating the activity of cerebral cortical pyramidal neurons optogenetically while the electroencephalogram, electromyogram, and cerebral lactate concentration are monitored. Experimental recordings are performed on cable-tethered mice while they undergo spontaneous sleep/wake cycles. Optogenetic equipment is assembled in our laboratory; recording equipment is commercially available.

Tags

Simultaneous Electroencephalography Real-time Measurement Lactate Concentration Optogenetic Manipulation Neuronal Activity Rodent Cerebral Cortex Glucose Utilization Non Rapid Eye Movement Sleep Cerebral Metabolism Slow Waves Electroencephalogram Depolarized/up State Hyperpolarized/down State Action Potentials
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter