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Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
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Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
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November 29, 2012
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An imaging technique for monitoring of membrane potential changes with sub-micrometer spatial and sub-millisecond temporal resolution is described. The technique, based on laser excitation of voltage-sensitive dyes, allows measurements of signals in axons and axon collaterals, terminal dendritic branches, and individual dendritic spines.
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Voltage-sensitive Dye RecordingAxonsDendritesDendritic SpinesIndividual NeuronsBrain SlicesBiophysical PropertiesFunctional OrganizationElectrical SignalsNerve CellElectrical PropertiesMeasurementsSubthreshold EventsAction Potential InitiationPatch-electrode ApproachImaging TechniquesParallel RecordingsMembrane Potential TransientsOrganic Voltage-sensitive Dyes (Vm-imaging)Sub-millisecond ResolutionSub-micrometer ResolutionVoltage-sensitive Molecular Probes
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Popovic, M., Gao, X., Zecevic, D. Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices. J. Vis. Exp. (69), e4261, doi:10.3791/4261 (2012).
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