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在GFP标记的下丘脑神经元的小鼠脑片成像钙反应

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

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在GFP标记的下丘脑神经元的小鼠脑片成像钙反应

DOI:

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09:14 min

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August 24, 2012

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Christian Schauer, Trese Leinders-Zufall

¹Department of Physiology, School of Medicine,University of Saarland, Homburg, Germany

Capitoli

00:05Titolo
01:52Solution Preparation and Brain Dissection
03:21Coronal Hypothalamic Sections Slicing
04:30Preparation of the Ca²⁺ Indicator Dye Loading Solution
06:35Microscopy and Analysis
07:59Results: Ca²⁺ Signals in _TGFP Neurons of Mouse Hypothalamic Brain Slices
08:52Conclusion

Summary

Traduzione automatica

在这个协议中，我们更新了最新进展，成像钙<sup> 2 +</sup>绿色荧光蛋白标记的神经元在脑组织切片，使用红色荧光钙信号<sup> 2 +</sup>指示剂染料。

Tags

Imaging Calcium Responses GFP-tagged Neurons Hypothalamic Mouse Brain Slices Encoding Of Information Molecular Steps Neurohormone Signal Transduction Cascades Intracellular Ca2+Synaptic Sites Green Fluorescent Protein (GFP)Spatially And Temporally Stimulus-induced Ca2+ Responses Non-green Fluorescent Ca2+ Indicator Dye Confocal Microscopy Ratiometric Ca2+ Indicator Fura-2 Ultraviolet (UV) Light

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