A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Stefan Schmollinger; Daniela Strenkert; Vittoria Offeddu; Andr&#233; Nordhues; Frederik Sommer; Michael Schroda

doi:10.3791/4083

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Een protocol voor de identificatie van eiwit-eiwit interacties op basis van 15 N Metabole Labeling, Immunoprecipitatie, kwantitatieve Massaspectrometrie en Affiniteit Modulatie

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

A Protocol for the Identification of Protein-protein Interactions Based on ¹⁵N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation