A Protocol for the Identification of Protein-protein Interactions Based on <sup>15</sup>N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Stefan Schmollinger; Daniela Strenkert; Vittoria Offeddu; Andr&#233; Nordhues; Frederik Sommer; Michael Schroda

doi:10.3791/4083

/

A Protocol for the Identification of Protein-protein Interactions Based on ¹⁵N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

A Protocol for the Identification of Protein-protein Interactions Based on ¹⁵N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation