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Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
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September 02, 2012
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HarvestingCryo-coolingCrystalsMembrane ProteinsLipidic MesophasesStructure DeterminationMacromolecular CrystallographyIntegral Membrane ProteinsMacromolecular X-ray CrystallographyMX Diffraction Quality CrystalsG Protein-coupled Receptor FieldIn Meso MethodLipidic Cubic Phase MethodTechnical ChallengesViscous NatureSticky NatureMicro-crystalsManipulating CrystalsGlass Sandwich Plates
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Li, D., Boland, C., Aragao, D., Walsh, K., Caffrey, M. Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography. J. Vis. Exp. (67), e4001, doi:10.3791/4001 (2012).
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