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In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos
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Neuroscienze
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JoVE Journal Neuroscienze
In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

DOI:
10.3791/3983-v

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07:53 min

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August 03, 2012

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, , ,
1Biology Department,University of Illinois at Springfield

Capitoli

  • 00:05Titolo
  • 01:35Embryonic Harvest
  • 02:32Electroporation of E11.5 Embryos
  • 04:17Culture of Embryos
  • 05:35Embryos for Analysis and Immunohistochemistry Analysis
  • 06:36Results: In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos
  • 07:27Conclusion

Summary

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This study describes the development of an in vitro electroporation technique that allows for the manipulation of gene expression in the lower rhombic lip of midgestation embryos.

Tags

In Vitro ElectroporationLower Rhombic LipMidgestation Mouse EmbryosNeuroepitheliumHindbrainNeural TubeRoofplateFourth VentricleUpper Rhombic LipCerebellumDiverse Neuronal Brainstem LineagesAuditory NeuronsCochlear NucleiPrecerebellar NucleiBalance And Motor ControlNeurogenesisTemporal WindowEmbryonic DaysGene Expression ConstructsLRL ProgenitorsFate Of NeuronsIn Utero Electroporation

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