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Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks
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Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks
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08:16 min
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February 21, 2012
DOI:
1Microbiology and Pathogenesis Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases,Centers for Disease Control and Prevention, 2Tick-Borne Diseases Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases,Centers for Disease Control and Prevention
Summary
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The collection of infected tick hemolymph, salivary glands, and saliva is important to study how tick-borne pathogens cause disease. In this protocol we demonstrate how to collect hemolymph and salivary glands from feeding Ixodes scapularis nymphs. We also demonstrate saliva collection from female I. scapularis adults.
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Cite this Article
Patton, T. G., Dietrich, G., Brandt, K., Dolan, M. C., Piesman, J., Gilmore Jr., R. D. Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks. J. Vis. Exp. (60), e3894, doi:10.3791/3894 (2012).
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