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Capitoli
- 00:05Titolo
- 03:50Prey- and Bait-protein Expression
- 05:19Normalization of the Bait Samples
- 08:21Prey Normalization
- 09:13AVEXIS Screening
- 10:43Representative Results
- 11:20Conclusion
AVEXIS er et højt gennemløb protein-interaktion assay udviklet til systematisk screening for hidtil ukendte ekstracellulære receptor-ligand-par involveret i cellulære genkendelse processer. Det er specielt designet til at detektere transiente proteininteraktioner, der er vanskelige at identificere ved hjælp af andre højt gennemløb fremgangsmåder.
Tags
Avidity-based Extracellular Interaction ScreeningAVEXISLow-affinity InteractionsExtracellular Receptor-ligand InteractionsIntercellular CommunicationMulticellular OrganismsProtein-protein InteractionsBinding PartnerMembrane-embedded ProteinsPosttranslational ModificationsInteraction AffinitiesHigh Throughput MethodsAVEXIS AssayWeak Protein InteractionsFalse Positive RateHigh Throughput FormatRecombinant Protein Libraries
