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表面等离子体共振检测到宿主细胞质的毒素易位
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
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表面等离子体共振检测到宿主细胞质的毒素易位

DOI:
10.3791/3686-v

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10:41 min

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January 03, 2012

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, , ,
1Department of Molecular Biology and Microbiology,University of Central Florida

Capitoli

  • 00:05Titolo
  • 01:31Cell Intoxication and Permeabilization
  • 03:34Sample and Standard Toxin Preparation
  • 06:11Surface Plasmon Resonance
  • 07:08Representative Surface Plasmon Resonance Traces and Analysis
  • 10:18Conclusion

Summary

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在这份报告中，我们描述了表面等离子体共振是如何用来检测输入到宿主细胞质中的毒素。胞浆内毒素量，这个高度敏感的方法可以提供定量数据，并可以应用到一系列的毒素。

Tags

Toxin TranslocationSurface Plasmon ResonanceAB ToxinsCell-binding B SubunitVesicle CarriersEndoplasmic Reticulum (ER)Catalytic A ChainProtein-conducting ChannelCytosolic TargetToxin TraffickingLysosomesGolgi ApparatusSubcellular Fractionation ProtocolDigitonin PermeabilizationAnti-toxin A Chain AntibodyDetection Method

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