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小鸡Statoacoustic神经节和脊髓植在胶原凝胶夹层和文化突起生长实验
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11:08 min
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December 20, 2011
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我们演示了如何解剖和文化小鸡E4 statoacoustic节和E6脊髓植。外植体无血清条件下24小时的三维胶原凝胶培养。突起的响应测试与生长因子补充介质与蛋白包被的磁珠。
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DissectionCultureChick Statoacoustic GanglionSpinal Cord ExplantsCollagen GelsNeurite Outgrowth AssaysSensory OrgansInner EarSAG NeuronsAxon Guidance CuesSurvival FactorsOtic Axons MisroutingGrowth FactorsNeurotrophin-3 (NT-3)Brain-derived Neurotrophic Factor (BDNF)Transgenic AnimalsIn Vitro MethodResponsiveness Of SAG NeuritesSoluble ProteinsMorphogensNeuron SurvivalEmbryonic Day 4 (E4)Three-dimensional Collagen Gels
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Cite this Article
Fantetti, K. N., Fekete, D. M. Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays. J. Vis. Exp. (58), e3600, doi:10.3791/3600 (2011).
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