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基因组编辑与的CompoZr自锌指核酸酶（ZFNs）
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June 14, 2012
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CompoZr自锌指核酸酶（ZFN）服务可在任何生物体或细胞系在由用户定义的任何轨迹的精确的基因组编辑。本文描述的CompoZr定制ZFN服务的设计，制造，验证和实施的过程。
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Genome EditingCompoZr Custom Zinc Finger NucleasesGene FunctionGenetic Basis Of DiseaseTraditional Gene Editing MethodsChemical-based MutagenesisRandom Integration Of DNA SequencesSynthetic SequencesAberrant Culture ConditionsTransient ZFN ExpressionPrecise Gene EditingHighly Efficient MannerZinc Finger NucleasesDouble-stranded DNA (dsDNA)CompoZr ZFN UnitDNA-cleavage DomainsDouble-strand Break (DSB)Non-homologous End-joining (NHEJ) DNA Repair PathwaySmall Insertions And Deletions (indels)FrameshiftFunctional Knockout
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Hansen, K., Coussens, M. J., Sago, J., Subramanian, S., Gjoka, M., Briner, D. Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs). J. Vis. Exp. (64), e3304, doi:10.3791/3304 (2012).
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