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Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
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June 14, 2012
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Summary
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The CompoZr Custom Zinc-Finger Nuclease (ZFN) Service enables precise genome editing in any organism or cell line at any locus defined by the user. This article describes the process for the design, manufacture, validation and implementation of the CompoZr Custom ZFN Service.
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Genome EditingCompoZr Custom Zinc Finger NucleasesGene FunctionGenetic Basis Of DiseaseTraditional Gene Editing MethodsChemical-based MutagenesisRandom Integration Of DNA SequencesSynthetic SequencesAberrant Culture ConditionsTransient ZFN ExpressionPrecise Gene EditingHighly Efficient MannerZinc Finger NucleasesDouble-stranded DNA (dsDNA)CompoZr ZFN UnitDNA-cleavage DomainsDouble-strand Break (DSB)Non-homologous End-joining (NHEJ) DNA Repair PathwaySmall Insertions And Deletions (indels)FrameshiftFunctional Knockout
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Hansen, K., Coussens, M. J., Sago, J., Subramanian, S., Gjoka, M., Briner, D. Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs). J. Vis. Exp. (64), e3304, doi:10.3791/3304 (2012).
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