This content is Free Access.
Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
26,807 Views
•
08:37 min
•
November 18, 2011
DOI:
Summary
Automatically generated
Microscopic imaging of live endothelial cells expressing GFP-actin allows characterization of dynamic changes in cytoskeletal structures. Unlike techniques that use fixed specimens, this method provides a detailed assessment of temporal changes in the actin cytoskeleton in the same cells before, during, and after various physical, pharmacological, or inflammatory stimuli.
Read Article
Cite this Article
Doggett, T. M., Breslin, J. W. Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin. J. Vis. Exp. (57), e3187, doi:10.3791/3187 (2011).
Copy