Rivista
/
Biologia
/
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

DOI:
10.3791/3155-v

•

06:00 min

•

November 18, 2011

•

, , , ,
1RIKEN BioResource Center

Capitoli

  • 00:05Titolo
  • 01:13Vitrification of 2-cell Mouse Embryos
  • 02:22Thawing Vitrified 2-cell Mouse Embryos
  • 05:03Representative Results
  • 05:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

An ethylene glycol-based vitrification method for mouse embryos is described. It is advantageous to other methods in its simplicity and low embryonic toxicity, and therefore can be broadly applicable to many strains of mice, including inbred and gene-modified mice.

Tags

CryopreservationMouse EmbryosEthylene Glycol-based VitrificationBiomedical SciencesGenetic ModificationsSlow Freezing MethodsVitrification MethodsCryoprotectantsToxicityEmbryo DevelopmentRIKEN BioResource CenterMouse StrainsGenetic BackgroundsPhenotypesVitrification TechniqueEmbryo SurvivalCryopreservation SolutionEthylene GlycolDMSOFicollSucrose

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Dissection of 6.5 dpc Mouse Embryos

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

Generating Chimeric Zebrafish Embryos by Transplantation

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

RNAi Interference by dsRNA Injection into Drosophila Embryos

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

Read Article