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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
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November 18, 2011
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An ethylene glycol-based vitrification method for mouse embryos is described. It is advantageous to other methods in its simplicity and low embryonic toxicity, and therefore can be broadly applicable to many strains of mice, including inbred and gene-modified mice.
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CryopreservationMouse EmbryosEthylene Glycol-based VitrificationBiomedical SciencesGenetic ModificationsSlow Freezing MethodsVitrification MethodsCryoprotectantsToxicityEmbryo DevelopmentRIKEN BioResource CenterMouse StrainsGenetic BackgroundsPhenotypesVitrification TechniqueEmbryo SurvivalCryopreservation SolutionEthylene GlycolDMSOFicollSucrose
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Mochida, K., Hasegawa, A., Taguma, K., Yoshiki, A., Ogura, A. Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification. J. Vis. Exp. (57), e3155, doi:10.3791/3155 (2011).
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