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Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis
Capitoli
- 00:05Titolo
- 01:22Fly Head Dissection
- 02:24Embedding and Arrangement in Molds
- 04:08Trimming and Sectioning
- 06:09Visualization of the Morphological Defects in the Adult Drosophila Eye
- 07:24Conclusion
A standard approach to prepare adult Drosophila eyes for semi-thin sectioning and light microscopic analysis is presented here. The protocol can be used for gross morphological analysis of eye defects, or with the indicated adjustments can be used to determine genetic requirements of genes in specific cell types of the eye (e.g. clonal analysis of photoreceptors) or for electron microscopic analysis.
