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高效的基因传递到多个中枢神经系统使用新界在子宫内电穿孔
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Neuroscienze
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JoVE Journal Neuroscienze
Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
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高效的基因传递到多个中枢神经系统使用新界在子宫内电穿孔

19,647 Views

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13:12 min

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June 23, 2011

DOI:

10.3791/2957-v

DOI:
10.3791/2957-v

•

13:12 min
June 23, 2011

•

4 Views
, , , , , ,
1Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children’s Hospital Research Institute,University of Calgary , 2Department of Medical Genetics, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute,University of Calgary

Tags

Gene DeliveryCNS TerritoriesIn Utero ElectroporationGene ExpressionDevelopmental PathwaysTransgenic TechnologiesLoss-of-function ModelsGain-of-function ModelsMouse TransgenicsAlternative MethodsLive Mouse EmbryosDNA InjectionElectrical CurrentCell MembranesEmbryonic Day (E) 11.5Ultrasound-guided Microinjection ProtocolE15.5 StageParietal Bone DifferentiationFrontal Bone DifferentiationRetina

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Cite this Article

Dixit, R., Lu, F., Cantrup, R., Gruenig, N., Langevin, L. M., Kurrasch, D. M., Schuurmans, C. Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation. J. Vis. Exp. (52), e2957, doi:10.3791/2957 (2011).

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