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Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
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Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

19,677 Views

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13:12 min

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June 23, 2011

DOI:

10.3791/2957-v

DOI:
10.3791/2957-v

•

13:12 min
June 23, 2011

•

4 Views
, , , , , ,
1Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children’s Hospital Research Institute,University of Calgary , 2Department of Medical Genetics, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute,University of Calgary

Summary

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In utero electroporation allows for rapid gene delivery in a spatially- and temporally-controlled manner in the developing central nervous system (CNS). Here we describe a highly adaptable in utero electroporation protocol that can be used to deliver expression constructs into multiple embryonic CNS domains, including the telencephalon, diencephalon and retina.

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Cite this Article

Dixit, R., Lu, F., Cantrup, R., Gruenig, N., Langevin, L. M., Kurrasch, D. M., Schuurmans, C. Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation. J. Vis. Exp. (52), e2957, doi:10.3791/2957 (2011).

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