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Entwicklung von Zell-spezifischen Anti-HIV-gp120-Aptamere für siRNA
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
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Entwicklung von Zell-spezifischen Anti-HIV-gp120-Aptamere für siRNA

19,335 Views

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13:47 min

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June 23, 2011

DOI:

10.3791/2954-v

DOI:
10.3791/2954-v

•

13:47 min
June 23, 2011

•

5 Views
, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Shared Resource-DNA/RNA Peptide,Beckman Research Institute of City of Hope

Summary

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Mehrere 2'-Fluoro RNA-Aptamere gegen HIV-1Ba-L gp120 mit Nanomol Affinität von einer RNA-Bibliothek isoliert durch<em> In-vitro-</em> SELEX Verfahren. Ein neuer Dual-hemmende Funktion anti-gp120 Aptamer-siRNA-Chimäre wird erstellt und zeigt viel versprechend für die systemische Anti-HIV-Therapie.

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Zhou, J., Li, H., Zhang, J., Piotr, S., Rossi, J. Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery. J. Vis. Exp. (52), e2954, doi:10.3791/2954 (2011).

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