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Ontwikkeling van Cell-type-specifieke anti-HIV-gp120 aptameren voor siRNA levering
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
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Ontwikkeling van Cell-type-specifieke anti-HIV-gp120 aptameren voor siRNA levering

19,335 Views

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13:47 min

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June 23, 2011

DOI:

10.3791/2954-v

DOI:
10.3791/2954-v

•

13:47 min
June 23, 2011

•

5 Views
, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Shared Resource-DNA/RNA Peptide,Beckman Research Institute of City of Hope

Summary

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Verschillende 2'-fluor RNA aptameren tegen HIV-1Ba-L gp120 met nanomole affiniteit geïsoleerd zijn van een RNA bibliotheek door<em> In vitro</em> SELEX procedure. Een nieuwe dual remmende functie anti-gp120 aptameer-siRNA chimera is gemaakt en toont grote belofte voor de systemische anti-HIV therapie.

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Zhou, J., Li, H., Zhang, J., Piotr, S., Rossi, J. Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery. J. Vis. Exp. (52), e2954, doi:10.3791/2954 (2011).

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