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Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

DOI:
10.3791/2889-v

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07:23 min

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June 09, 2011

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, , , ,
1Center for Vector Biology and Zoonotic Diseases, Department of Environmental Sciences,The Connecticut Agricultural Experiment Station

Capitoli

  • 00:05Titolo
  • 01:13Mosquito Sorting and Collection
  • 02:39Homogenization of Mosquitoes
  • 03:25Preparation of Vero Cells
  • 04:49Inoculation of Vero cells with Mosquito Pool Homogenates
  • 05:38Screening Vero Cells for Virus Growth
  • 06:40Viruses Detected in Field-collected Mosquitoes by Vero Cell Culture Assay
  • 07:05Conclusion

Summary

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We describe a method to process and screen field-collected mosquitoes for a diversity of viruses by Vero cell culture assay. By employing this technique, we have detected 9 different viruses from 4 taxonomic families in mosquitoes collected in Connecticut.

Tags

Infectious VirusField-collected MosquitoesVero Cell Culture AssayWest Nile VirusDengue VirusChickungunya VirusSurveillance And Control ProgramsViral InfectionTrap LocationSpecies GroupingPooled SpecimensBuffered SalineMixer-millVero Cell CulturesCytopathic EffectDiagnostic AssaysVirus IsolationConnecticut USAHuman DiseaseNew Records

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