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Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
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Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
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June 09, 2011
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We describe a method to process and screen field-collected mosquitoes for a diversity of viruses by Vero cell culture assay. By employing this technique, we have detected 9 different viruses from 4 taxonomic families in mosquitoes collected in Connecticut.
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Infectious VirusField-collected MosquitoesVero Cell Culture AssayWest Nile VirusDengue VirusChickungunya VirusSurveillance And Control ProgramsViral InfectionTrap LocationSpecies GroupingPooled SpecimensBuffered SalineMixer-millVero Cell CulturesCytopathic EffectDiagnostic AssaysVirus IsolationConnecticut USAHuman DiseaseNew Records
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Armstrong, P. M., Andreadis, T. G., Finan, S. L., Shepard, J. J., Thomas, M. C. Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay. J. Vis. Exp. (52), e2889, doi:10.3791/2889 (2011).
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