Rivista
/
Immunologia e infezioni
/
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
JoVE Journal
Immunologia e infezioni
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

DOI:
10.3791/2820-v

•

11:29 min

•

December 03, 2011

•

, , , , ,
1Laboratory of Water and Food Viral Pollution, Department of Microbiology, Faculty of Biology,University of Barcelona

Capitoli

  • 00:05Titolo
  • 00:11Introduction
  • 01:56Collection of Water Samples
  • 02:45Viral Concentration by Organic Flocculation
  • 06:10Nucleic Acid Extraction and Quantification of Viruses
  • 10:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

The study describes a cost-effective method for the identification of the source of fecal/urine contamination or contamination by nitrates in water using qPCR for the specific quantification of human/porcine/bovine DNA viruses, adenoviruses and polyomaviruses, proposed as MST tools.

Tags

Microbial Source TrackingCost-effective MethodSpecific Human VirusesSpecific Animal VirusesViral IndicatorsFecal ContaminationWater QualityMolecular AssaysAdenovirusesPolyomavirusesQuantitative PCR (qPCR)Porcine AdenovirusesBovine PolyomavirusesWater Sample AnalysisNitrates

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Using Luciferase to Image Bacterial Infections in Mice

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells

VirWaTest, A Point-of-Use Method for the Detection of Viruses in Water Samples

Read Article