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A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti
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A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti
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May 16, 2011
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Summary
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A simplified yet accurate method to collect and stain mosquito hemocytes is described. Our method combines the simplicity of perfusion with the accuracy of high injection techniques to isolate clean preparations of hemocytes in Aedes mosquitoes. This method facilitates studies requiring knowledge of the types of hemocytes and their abundance.
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Qayum, A. A., Telang, A. A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti. J. Vis. Exp. (51), e2772, doi:10.3791/2772 (2011).
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