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高效转染树突状细胞的无细胞的成熟优化的协议
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
DOI:

08:08 min

July 08, 2011

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Capitoli

  • 00:05Titolo
  • 01:05Program the Amaxa 96-well Shuttle Nucleofactor
  • 02:00Prepare DCs for Transfection
  • 03:40Transfect DCs
  • 05:07Infection and Harvest of Cells with Newcastle Disease Virus
  • 06:09siRNA Gene Targeting Results in Drastic Reduction of Message and Total Reduction of Protein Expression in Monocyte DCs
  • 07:51Conclusion

Summary

Traduzione automatica

我们目前我们优化的高通量nucleofection协议,要么质粒DNA或不会造成细胞的成熟的siRNA转染初级人类单核细胞衍生的树突状细胞的有效方法。我们进一步提供证据证明为成功的siRNA沉默靶基因的mRNA和蛋白水平的RIG - I。

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