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Genetik ve epigenetik Analizleri Parafin Gömülü Malzeme DNA Ekstraksiyon
JoVE Journal
Biologia
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JoVE Journal Biologia
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
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Genetik ve epigenetik Analizleri Parafin Gömülü Malzeme DNA Ekstraksiyon

55,344 Views

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13:32 min

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March 26, 2011

DOI:

10.3791/2763-v

DOI:
10.3791/2763-v

•

13:32 min
March 26, 2011

•

28 Views
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1Department of Integrative Oncology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Photography/Video Production, Multi-Media Services,BC Cancer Agency, 4Department of Pathology and Laboratory Medicine,University of British Columbia – UBC

Summary

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Bu video formalin ile fikse parafine gömülü malzeme DNA ekstraksiyonu için protokol göstermektedir. Bu doku kesitlerinde ksilen ile deparafinize olduğu bir çok günlük bir prosedür, etanol ile sulandırılır ve sonraki gen-spesifik veya genom analizi için DNA arındırmak ve izole etmek için proteinaz K ile tedavi edilir.

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Pikor, L. A., Enfield, K. S. S., Cameron, H., Lam, W. L. DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses. J. Vis. Exp. (49), e2763, doi:10.3791/2763 (2011).

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