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DNA-extractie van paraffine Embedded materiaal voor genetische en epigenetische Analyses
JoVE Journal
Biologia
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JoVE Journal Biologia
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
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DNA-extractie van paraffine Embedded materiaal voor genetische en epigenetische Analyses

55,344 Views

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13:32 min

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March 26, 2011

DOI:

10.3791/2763-v

DOI:
10.3791/2763-v

•

13:32 min
March 26, 2011

•

28 Views
, , ,
1Department of Integrative Oncology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Photography/Video Production, Multi-Media Services,BC Cancer Agency, 4Department of Pathology and Laboratory Medicine,University of British Columbia – UBC

Summary

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Deze video toont het protocol voor DNA-extractie van formaline gefixeerde in paraffine ingebedde materiaal. Dit is een multi-dagen procedure waarbij weefsel secties worden ontdaan met xyleen, gerehydrateerd met ethanol en behandeld met proteinase K te zuiveren en DNA te isoleren voor latere gen-specifieke of genome-wide analyse.

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Cite this Article

Pikor, L. A., Enfield, K. S. S., Cameron, H., Lam, W. L. DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses. J. Vis. Exp. (49), e2763, doi:10.3791/2763 (2011).

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