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Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
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Capitoli
- 00:05Titolo
- 01:14Infection of CD4+ T-Cells with HIV-1
- 02:42Purification of HIV-1 Infected Cells
- 03:41CD107a Degranulation Assay
- 06:4151Cr Release Assay
- 10:07Evaluation of CD107a Degranulation and NK Cell Cytotoxicity
- 11:45Conclusion
Cytotoxicity assays to measure natural killer cell lytic responses to HIV-infected cells is limited by the purity of the target cells. We demonstrate here the isolation of a highly purified population of HIV-1 infected primary T-cell blasts by taking advantage of HIV-1 s ability to down-modulate CD4.
