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Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
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May 29, 2011
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We will describe a method which measures the kinetics of ion transport of membrane proteins alongside site-specific analysis of conformational changes using fluorescence on single cells. This technique is adaptable to ion channels, transporters and ion pumps and can be utilized to determine distance constraints between protein subunits.
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Richards, R., Dempski, R. E. Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling. J. Vis. Exp. (51), e2627, doi:10.3791/2627 (2011).
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