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Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
JoVE Journal
Biologia
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JoVE Journal Biologia
Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

35,047 Views

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10:58 min

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August 22, 2007

DOI:

10.3791/261-v

DOI:
10.3791/261-v

•

10:58 min
August 22, 2007

•

4 Views
, , , , , ,
1Department of Biomedical Engineering,University of California, Irvine (UCI), 2Stem Cell Research Center,University of California, Irvine (UCI), 3Institute for Brain Aging and Dementia,University of California, Irvine (UCI)

Summary

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In this video we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to farbricate a microfluidic device for culturing neurons.

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Microfluidic DeviceNeuron CulturingSoft LithographyPDMS FabricationSilicon WaferPhotolithographyMaster MoldNegative MoldReservoirsCover Glass BondingSterilization

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Harris, J., Lee, H., Vahidi, B., Tu, C., Cribbs, D., Jeon, N. L., Cotman, C. Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons. J. Vis. Exp. (7), e261, doi:10.3791/261 (2007).

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