In this video we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to farbricate a microfluidic device for culturing neurons.
Harris, J., Lee, H., Vahidi, B., Tu, C., Cribbs, D., Jeon, N. L., Cotman, C. Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons. J. Vis. Exp. (7), e261, doi:10.3791/261 (2007).