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Zymography Fonksiyonel Matrix Metalloproteinazlar Tespiti
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November 08, 2010
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Bu protokol, kültür süpernatantlar veya vücut sıvıları matriks metalloproteinazların tespit etmek için bir faaliyet tabanlı test açıklamaktadır.
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Functional Matrix MetalloproteinasesZymographyMMPsZinc-containing EndopeptidasesPeptide BondsCollagenasesGelatinasesMT-MMPStromelysinsMatrilysinsCell InvasionCartilage DegradationWound HealingEmbryogenesisRheumatoid ArthritisCancerChronic Obstructive Pulmonary DiseaseMMP-2Gelatinase AType IV CollagenaseCell Culture SupernatantsSemi-quantitative TechniquePolyacrylamide GelSodium Dodecyl Sulfate (SDS)Gelatin Substrate
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Hu, X., Beeton, C. Detection of Functional Matrix Metalloproteinases by Zymography. J. Vis. Exp. (45), e2445, doi:10.3791/2445 (2010).
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