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Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
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JoVE Journal Neuroscienze
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells

Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells

DOI:
10.3791/2405-v

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04:55 min

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February 23, 2011

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, ,
1Department of Molecular Genetics and Molecular Biology ,Duke University, 2Department of Chemistry,Duke University

Capitoli

  • 00:05Titolo
  • 01:25Transfection of HEK293T Cells and Plating for Surface Expression Assay
  • 02:47Cell Surface Staining of Receptors
  • 04:12Cell Surface Staining of the Odorant Receptor Olfr62
  • 04:27Conclusion

Summary

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Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or GPCRs.

Tags

AssayingSurface ExpressionChemosensory ReceptorsHeterologous CellsOlfactionG Protein Coupled Receptors (GPCRs)Odorant MoleculesIntracellular SignalG Protein GαolfCombinatorial RuleBiochemical StudiesLigand Binding StudiesHeterologous CellsReceptor Transporting Protein (RTP)Olfactory Sensory NeuronsChaperonesTransfectionBiochemical AssaysCell LineLive Cell Staining

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