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An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
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An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
Capitoli
- 00:05Titolo
- 01:01Introduction
- 01:33Preparation of Solutions
- 02:29Isolation of Retina from Mouse Eye
- 04:08Treatment of Retina with Enzyme MIxture and Mounting in Recording Chamber
- 06:10Localization of EGFP Expressing Ganglion Cells and Light Stimulation
- 09:51Analysis of Anatomy of Recorded Ganglion Cells
- 11:21Response of Retinal Ganglion Cell to Light Stimulation and Correlation with Morphology
- 12:45Conclusion
This article provides a description of how to dissect and record from the isolated retinal preparation in mouse. In particular, we describe how to record light responses from a fluorescently labeled ganglion cell population and subsequently identify and analyze its morphology.
Tags
Isolated Retinal PreparationLight ResponseGenetically Labeled Retinal Ganglion CellsVertebrate VisionRod And Cone PhotoreceptorsON And OFF PathwaysBipolar Cells (BCs)Retinal Ganglion Cells (RGCs)Inner Plexiform Layer (IPL)Excitatory InputIlluminationParallel SignalingBrain Visual NucleiFluorescent Reporter Mouse LinesRGC SubtypesElectrophysiological Recording
