Recording of Visual Evoked Potentials via the Skull Electrodes in a Rat Model
Trascrizione
After anesthetizing the animal and preparing the skin as before, transfer it to a dark room. There, maintain its body temperature within half a degree of 37 degrees Celsius using a homeothermic blanket system and a temperature probe. While being monitored, dark-adapt the animal for 5 to 30 minutes.
After half an hour, dilate the pupils with 1% tropicamide eyedrops. Then, using scissors and forceps, open the skin over the skull, and access the pre-placed di situ screw electrodes. Connect the screws to the impedance meter. Then, measure and maintain the electrode impedance below 5 kiloohms.
Now, connect the screw over the contralateral visual cortex of the stimulated eye. Next, insert a needle electrode into the tail to serve as the ground. Now, place a calibrated mini-Ganzfeld stimulator onto the skin around the eyelids to improve the eye isolation. Then, deliver photic stimulation using 100 flashes at 1 hertz, with low-pass filter at 1 hertz, and the high-band pass filter at 100 hertz.
