Stimulation of Patterned Neuronal Cultures by a Magnetic Field
Trascrizione
Take a patterned coverslip with a neuronal ring culture within a culture plate containing an extracellular recording solution.
These cells are preincubated with a calcium-sensitive fluorescent dye to measure intracellular calcium levels.
Place the plate on a fluorescence microscope stage.
Position the circular magnetic coil connected to a magnetic stimulator concentrically at a suitable distance above the culture to ensure optimal stimulation.
Using the magnetic stimulator, apply high voltage and current through the coil to generate a time-varying magnetic field.
This magnetic field induces an electric field in the neurons, generating an action potential.
Upon reaching the nerve terminal, the action potential activates the voltage-gated calcium channels, allowing calcium influx into the cell and neurotransmitter release at the synapse.
This release facilitates signal transmission to the neighboring neurons through synaptic connections.
Due to the calcium ion influx, these ions bind to the intracellular calcium-sensitive dye, increasing fluorescence indicative of neuronal activity.
