Electrophysiological Recordings For Assessing Neuronal Regenerations in Co-cultured Spinal Cord Slices
Trascrizione
Take a polymer-coated multi-electrode array or MEA, with co-cultured embryonic spinal cord slices positioned in two separate zones, allowing for simultaneous recording from both slices.
The slice shows regenerated neural connections in the previously lesioned area.
Place the MEA in a recording chamber containing an extracellular solution rich in ions and nutrients to maintain neuronal survival.
Allow the system to stabilize, facilitating neuron adaptation and communication.
Neurons communicate via electrical signals called action potentials, which are recorded by the nearby electrodes in the MEA.
Individual signals from each electrode are combined, producing an electrical signal from each slice.
Regularly replace the extracellular solution to maintain neuron stability.
Add an extracellular solution with inhibitors that block inhibitory neurotransmitter receptors on neuronal membranes, keeping neurons active for a longer duration.
Compare the electrical activity from both slices. A synchronized electrical signal between the two slices confirms the synaptic connections and successful neural regeneration.
