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Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
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JoVE Journal Neuroscienze
Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

DOI:
10.3791/2261-v

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11:02 min

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May 12, 2011

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, ,
1Department of Surgery,University of Toronto

Capitoli

  • 00:05Titolo
  • 01:13Introduction
  • 01:44Syringe Preparation for Intraocular Injections
  • 04:48Intraocular Injection Procedure: Targeting the Retina Globally
  • 06:50Selectively Targeting Retinal Ganglion Cells from the Optic Nerve Stump
  • 09:28RGC Targeting by Injection of the Transected Optic Nerve Stump
  • 10:31Conclusion

Summary

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Optic Nerve transection is a widely used model of adult CNS injury. This model is ideal for performing a number of experimental manipulations that target the retina globally or directly target the injured neuronal population of retinal ganglion cells.

Tags

Optic Nerve TransectionExperimental ManipulationsMammalian CNSRetinal Ganglion CellsOptic NerveAxotomizedApoptotic Neuronal Cell DeathVitreous ChamberDrug DeliveryIntraocular InjectionsViral VectorsPlasmidsSiRNAsAdeno-Associated Virus (AAV) VectorsRGCs TargetingSuperior ColliculusApoptotic Mechanisms

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