È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:00Titolo
- 00:29Method Overview
- 01:12Introduction
- 02:30Plasmid Preparation for Electroporation
- 03:31Glass Needle Preparation
- 04:07Microinjector Assembling
- 05:25Anaesthetizing Animal
- 06:42Surgery, Microinjection, Electroporation
- 09:22Risultati
- 10:19Conclusion
Tags
Gene DeliveryPostnatal Rat BrainNon-ventricularPlasmid InjectionElectroporationTransgenic AnimalsGene Of InterestKnockout AnimalsCompensatory MechanismsGene Expression TimingTransfected CellsMicroinjectionIn Vivo ElectroporationTargeted DeliverySiRNADye MoleculesNewborn Rodent BrainNon-migratory Cell TypesTwo-photon In Vivo ImagingElectrophysiological Experiments
