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Fluorescein Staining in an Autoimmune Dry Eye Rat Model to Examine Corneal Damage

Fluorescein Staining in an Autoimmune Dry Eye Rat Model to Examine Corneal Damage

Trascrizione

Use fluorescein scanning to measure corneal damage by adding 2 microliters of 0.2% fluorescein to the rat cornea. With a gloved finger, passively open and close the rat eyelids three times to spread the fluorescein dye on the surface of the eye.

After 1 minute, use a 3-milliliter syringe to draw 1 milliliter of saline. Then, position the syringe about 2 to 3 millimeters anterior to the cornea, and gently apply saline onto the eye. With the background lights switched off, acquire images under the cobalt blue filter of an ocular imaging microscope.

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