Intraduodenal Cannulation in a Rat Model for Immunopathology Studies

To cannulate the duodenum, first, locate the tissue as the bright pink section of the small intestine, which upon gentle downward pulling reveals the stomach. Then, use a sterile 23-gauge needle to make a small puncture hole in the duodenum at the pylorus, approximately 2 centimeters below the junction of the stomach and duodenum. Next, insert the J-shaped hook end of the duodenal cannula through the puncture and secure the cannula in place with a drop of cyanoacrylate glue. Then, use sutures to close the abdominal muscle wall incision.