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Adenovirus-vermittelte genetische Entfernung von Signalmolekülen in kultivierten primären embryonalen Maus-Fibroblasten
JoVE Journal
Biologia
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JoVE Journal Biologia
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
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Adenovirus-vermittelte genetische Entfernung von Signalmolekülen in kultivierten primären embryonalen Maus-Fibroblasten

24,623 Views

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11:00 min

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September 09, 2010

DOI:

10.3791/2160-v

DOI:
10.3791/2160-v

•

11:00 min
September 09, 2010

•

2 Views
, ,
1Department of Molecular and Cellular Biology,University of Guelph

Summary

Automatically generated

In diesem Video verwenden wir ein Adenovirus Durchführung der Cre-Rekombinase-Gen zur primären embryonalen Maus-Fibroblasten Tragen einer floxed Rac1 Allel zu infizieren.

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Adenovirus-mediatedGenetic RemovalSignaling MoleculesCultured Primary Mouse Embryonic FibroblastsCre-loxP SystemConditional DeletionCre RecombinaseFloxed DNARecombination EventExcisionAdenovirusCre Recombinase GeneInfectPrimary Mouse Embryonic Fibroblasts (MEFs)Floxed Rac1 AlleleMorphological ChangesActin CytoskeletonViral TransductionCre ExpressionStained Using Phalloidin DyeConfocal Laser Scanning Microscopy

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Cite this Article

Hawley, S. P., Wills, M. K. B., Jones, N. Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts. J. Vis. Exp. (43), e2160, doi:10.3791/2160 (2010).

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