UV-Visible Spectroscopy for Monitoring Fibrin Clot Formation

Begin by adding 1.5 micromolar of freshly-prepared fibrinogen in 200 microliters of clot formation buffer to each Aβ negative 42-containing and control wells, and incubate the plate on a rotating platform at room temperature. After 30 minutes, simultaneously add 30 microliters of freshly-prepared thrombin solution directly into the center of each fibrinogen-containing well to initiate clot formation, and immediately read the absorbance of the in-vitro clots at 350 nanometers, repeating the measurement every 30 to 60 seconds over the course of 10 minutes.