/
/
Identifying Dysregulated Genes Induced by Kaposi’s Sarcoma-associated Herpesvirus (KSHV)
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Identifying Dysregulated Genes Induced by Kaposi’s Sarcoma-associated Herpesvirus (KSHV)
12,429 Views
•
07:02 min
•
September 14, 2010
DOI:
Summary
Automatically generated
Host cell factors play a critical role in the establishment and maintenance of Kaposi's sarcoma (KS). We outline methods to identify host cell factors altered in KSHV-infected DMVEC cells, and in KS tumor tissue. Cellular genes altered by virus will serve as potential target(s) for novel therapeutics.
Explore More Videos
Kaposi’s Sarcoma-associated HerpesvirusDysregulated GenesKSHVHIV/AIDSMalignancySouthern AfricaAngioproliferative TumorVascular Endothelial CellsB Cell Lymphoproliferative DiseasesPleural Effusion LymphomasMulticentric Castleman’s DiseaseLytic ReplicationOncogenesisTumor ProgressionHost-factor DeterminantsPrognostic MarkersMetastasisTherapeuticsDermal Microvascular Endothelial Cells (DMVEC)Microarray AnalysisProtein ExpressionImmunoblotDual Labeled ImmunofluorescenceTranscriptional ExpressionQuantitative Real-time Polymerase Chain Reaction (qRT-PCR)
Read Article
Cite this Article
Alcendor, D., Knobel, S. Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV). J. Vis. Exp. (43), e2078, doi:10.3791/2078 (2010).
Copy