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Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
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Capitoli
- 00:06Titolo
- 00:48Introduction
- 01:15Reagent Preparation
- 01:56Cell Preparation
- 03:11Calpain Assay in Fixed Cells
- 04:48Data Acquisition (Fixed Cells)
- 06:55Calpain Assay in Live Cells
- 08:22Calpain Assay in Mixed Cells
- 10:05Analysis and Representative Results
- 11:09Conclusion
Tags
Calpain ActivityFlow CytometryFixed CellsLiving CellsCalcium-sensitive ProteasesPhysiological ProcessesSignalingCytoskeletal RemodelingGene Expression RegulationApoptosisCell Cycle ProgressionPathologiesMuscular DystrophiesCancerDiabetesAlzheimer’s DiseaseMultiple SclerosisCalpain RegulationMRNA LevelsProtein LevelsGene Expression TechniquesFluorescent Substrate BOC-LM-CMACCalpain Activity MeasurementMurine 32Dkit Leukemia CellsSplenocyte Population
